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rabbit polyclonal mgat3  (Abcam)


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    Structured Review

    Abcam rabbit polyclonal mgat3
    Schema of <t>MGAT3</t> and MGAT5-mediated N-glycosylation. Bisecting GlcNAc blocks glycans branching via MGAT5.
    Rabbit Polyclonal Mgat3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 20243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+mgat3/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc06534161-63-6-10
    Average 99 stars, based on 20243 article reviews
    rabbit polyclonal mgat3 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER"

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    doi:

    Schema of MGAT3 and MGAT5-mediated N-glycosylation. Bisecting GlcNAc blocks glycans branching via MGAT5.
    Figure Legend Snippet: Schema of MGAT3 and MGAT5-mediated N-glycosylation. Bisecting GlcNAc blocks glycans branching via MGAT5.

    Techniques Used:

    (A) MGAT3 and MGAT5 W-B of the cell lysate from LNCaP (c-26) and (c-86) cells; β-actin was used as a loading control. (B) Matriptase W-B of the plasma membrane (PM) fractions from LNCaP (c-26) and (c-86) cells; the PM samples were normalized by E-cadherin. (C) The L-PHA lectin W-B of Matriptase IP from LNCaP (c-26) and (c-86) cells. The bottom panel represents the input normalized for the Matriptase.
    Figure Legend Snippet: (A) MGAT3 and MGAT5 W-B of the cell lysate from LNCaP (c-26) and (c-86) cells; β-actin was used as a loading control. (B) Matriptase W-B of the plasma membrane (PM) fractions from LNCaP (c-26) and (c-86) cells; the PM samples were normalized by E-cadherin. (C) The L-PHA lectin W-B of Matriptase IP from LNCaP (c-26) and (c-86) cells. The bottom panel represents the input normalized for the Matriptase.

    Techniques Used:

    Immunostaining of GM130 (green) and MGAT3 (red) (A), and giantin (green) and MGAT5 (red) (B) in the LNCaP cells: control and treated with 35 mM EtOH for 96 h. Nuclei were counterstained with DAPI (blue). All confocal images were acquired with the same imaging parameters; bars, 10 μm. (C) Quantification of Pearson’s overlap coefficient for indicated proteins for cells from A and B; means ± SD; * - p<0.001.
    Figure Legend Snippet: Immunostaining of GM130 (green) and MGAT3 (red) (A), and giantin (green) and MGAT5 (red) (B) in the LNCaP cells: control and treated with 35 mM EtOH for 96 h. Nuclei were counterstained with DAPI (blue). All confocal images were acquired with the same imaging parameters; bars, 10 μm. (C) Quantification of Pearson’s overlap coefficient for indicated proteins for cells from A and B; means ± SD; * - p<0.001.

    Techniques Used: Immunostaining, Imaging

    (A) MGAT3 and MGAT5 W-B of the lysate from LNCaP cells: control and treated with 35 mM EtOH for 96 h. (B) Integrin α5 W-B of the plasma membrane (PM) fraction from the cells presented in A; the PM samples were normalized by E-cadherin
    Figure Legend Snippet: (A) MGAT3 and MGAT5 W-B of the lysate from LNCaP cells: control and treated with 35 mM EtOH for 96 h. (B) Integrin α5 W-B of the plasma membrane (PM) fraction from the cells presented in A; the PM samples were normalized by E-cadherin

    Techniques Used:

    (A) 3D SIM reconstruction of giantin (green) and MGAT3 (red) IF in normal prostate and tissue section of PCa patients (Gleason 7); bars, 5 µm. White boxes indicate area enlarged below. (B) Quantification of colocalization of giantin and MGAT3 from the sections in A; 10 patients for each group were observed; means ± SD; * - p<0.001.
    Figure Legend Snippet: (A) 3D SIM reconstruction of giantin (green) and MGAT3 (red) IF in normal prostate and tissue section of PCa patients (Gleason 7); bars, 5 µm. White boxes indicate area enlarged below. (B) Quantification of colocalization of giantin and MGAT3 from the sections in A; 10 patients for each group were observed; means ± SD; * - p<0.001.

    Techniques Used:

    The working model of alcohol effect on N-glycosylation in PCa. In low aggressive PCa cells, pro-metastatic proteins are modified by MGAT3, which reduces their binding to the Gal-3, thus blocking their stabilization and retention at the cell surface. In alcohol-treated PCa cells, Golgi is fragmented and domination of MGAT5-mediated glycosylation promotes plasma membrane expression of proteins via their strong interaction with Gal-3. This, in turn, facilitates PCa progression.
    Figure Legend Snippet: The working model of alcohol effect on N-glycosylation in PCa. In low aggressive PCa cells, pro-metastatic proteins are modified by MGAT3, which reduces their binding to the Gal-3, thus blocking their stabilization and retention at the cell surface. In alcohol-treated PCa cells, Golgi is fragmented and domination of MGAT5-mediated glycosylation promotes plasma membrane expression of proteins via their strong interaction with Gal-3. This, in turn, facilitates PCa progression.

    Techniques Used: Modification, Binding Assay, Blocking Assay, Expressing



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    rabbit polyclonal mgat3  (Abcam)
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    Abcam rabbit polyclonal mgat3
    Schema of <t>MGAT3</t> and MGAT5-mediated N-glycosylation. Bisecting GlcNAc blocks glycans branching via MGAT5.
    Rabbit Polyclonal Mgat3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+mgat3/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc06534161-63-6-10
    Average 99 stars, based on 1 article reviews
    rabbit polyclonal mgat3 - by Bioz Stars, 2026-10
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    Image Search Results


    Schema of MGAT3 and MGAT5-mediated N-glycosylation. Bisecting GlcNAc blocks glycans branching via MGAT5.

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    doi:

    Figure Lengend Snippet: Schema of MGAT3 and MGAT5-mediated N-glycosylation. Bisecting GlcNAc blocks glycans branching via MGAT5.

    Article Snippet: The primary antibodies used were: a) rabbit polyclonal – MGAT3 (Abcam, ab103427), MGAT5 (Abcam, ab68595), and ST14 (Matriptase) (Abcam, ab28266); b) mouse monoclonal – E-cadherin (Abcam, ab1416), β-actin (Sigma, A2228), integrin alpha V + beta 6 (Abcam, ab77906), and giantin (Abcam, ab37266); c) mouse polyclonal – GM130 (Abcam, ab169276).

    Techniques:

    (A) MGAT3 and MGAT5 W-B of the cell lysate from LNCaP (c-26) and (c-86) cells; β-actin was used as a loading control. (B) Matriptase W-B of the plasma membrane (PM) fractions from LNCaP (c-26) and (c-86) cells; the PM samples were normalized by E-cadherin. (C) The L-PHA lectin W-B of Matriptase IP from LNCaP (c-26) and (c-86) cells. The bottom panel represents the input normalized for the Matriptase.

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    doi:

    Figure Lengend Snippet: (A) MGAT3 and MGAT5 W-B of the cell lysate from LNCaP (c-26) and (c-86) cells; β-actin was used as a loading control. (B) Matriptase W-B of the plasma membrane (PM) fractions from LNCaP (c-26) and (c-86) cells; the PM samples were normalized by E-cadherin. (C) The L-PHA lectin W-B of Matriptase IP from LNCaP (c-26) and (c-86) cells. The bottom panel represents the input normalized for the Matriptase.

    Article Snippet: The primary antibodies used were: a) rabbit polyclonal – MGAT3 (Abcam, ab103427), MGAT5 (Abcam, ab68595), and ST14 (Matriptase) (Abcam, ab28266); b) mouse monoclonal – E-cadherin (Abcam, ab1416), β-actin (Sigma, A2228), integrin alpha V + beta 6 (Abcam, ab77906), and giantin (Abcam, ab37266); c) mouse polyclonal – GM130 (Abcam, ab169276).

    Techniques:

    Immunostaining of GM130 (green) and MGAT3 (red) (A), and giantin (green) and MGAT5 (red) (B) in the LNCaP cells: control and treated with 35 mM EtOH for 96 h. Nuclei were counterstained with DAPI (blue). All confocal images were acquired with the same imaging parameters; bars, 10 μm. (C) Quantification of Pearson’s overlap coefficient for indicated proteins for cells from A and B; means ± SD; * - p<0.001.

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    doi:

    Figure Lengend Snippet: Immunostaining of GM130 (green) and MGAT3 (red) (A), and giantin (green) and MGAT5 (red) (B) in the LNCaP cells: control and treated with 35 mM EtOH for 96 h. Nuclei were counterstained with DAPI (blue). All confocal images were acquired with the same imaging parameters; bars, 10 μm. (C) Quantification of Pearson’s overlap coefficient for indicated proteins for cells from A and B; means ± SD; * - p<0.001.

    Article Snippet: The primary antibodies used were: a) rabbit polyclonal – MGAT3 (Abcam, ab103427), MGAT5 (Abcam, ab68595), and ST14 (Matriptase) (Abcam, ab28266); b) mouse monoclonal – E-cadherin (Abcam, ab1416), β-actin (Sigma, A2228), integrin alpha V + beta 6 (Abcam, ab77906), and giantin (Abcam, ab37266); c) mouse polyclonal – GM130 (Abcam, ab169276).

    Techniques: Immunostaining, Imaging

    (A) MGAT3 and MGAT5 W-B of the lysate from LNCaP cells: control and treated with 35 mM EtOH for 96 h. (B) Integrin α5 W-B of the plasma membrane (PM) fraction from the cells presented in A; the PM samples were normalized by E-cadherin

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    doi:

    Figure Lengend Snippet: (A) MGAT3 and MGAT5 W-B of the lysate from LNCaP cells: control and treated with 35 mM EtOH for 96 h. (B) Integrin α5 W-B of the plasma membrane (PM) fraction from the cells presented in A; the PM samples were normalized by E-cadherin

    Article Snippet: The primary antibodies used were: a) rabbit polyclonal – MGAT3 (Abcam, ab103427), MGAT5 (Abcam, ab68595), and ST14 (Matriptase) (Abcam, ab28266); b) mouse monoclonal – E-cadherin (Abcam, ab1416), β-actin (Sigma, A2228), integrin alpha V + beta 6 (Abcam, ab77906), and giantin (Abcam, ab37266); c) mouse polyclonal – GM130 (Abcam, ab169276).

    Techniques:

    (A) 3D SIM reconstruction of giantin (green) and MGAT3 (red) IF in normal prostate and tissue section of PCa patients (Gleason 7); bars, 5 µm. White boxes indicate area enlarged below. (B) Quantification of colocalization of giantin and MGAT3 from the sections in A; 10 patients for each group were observed; means ± SD; * - p<0.001.

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    doi:

    Figure Lengend Snippet: (A) 3D SIM reconstruction of giantin (green) and MGAT3 (red) IF in normal prostate and tissue section of PCa patients (Gleason 7); bars, 5 µm. White boxes indicate area enlarged below. (B) Quantification of colocalization of giantin and MGAT3 from the sections in A; 10 patients for each group were observed; means ± SD; * - p<0.001.

    Article Snippet: The primary antibodies used were: a) rabbit polyclonal – MGAT3 (Abcam, ab103427), MGAT5 (Abcam, ab68595), and ST14 (Matriptase) (Abcam, ab28266); b) mouse monoclonal – E-cadherin (Abcam, ab1416), β-actin (Sigma, A2228), integrin alpha V + beta 6 (Abcam, ab77906), and giantin (Abcam, ab37266); c) mouse polyclonal – GM130 (Abcam, ab169276).

    Techniques:

    The working model of alcohol effect on N-glycosylation in PCa. In low aggressive PCa cells, pro-metastatic proteins are modified by MGAT3, which reduces their binding to the Gal-3, thus blocking their stabilization and retention at the cell surface. In alcohol-treated PCa cells, Golgi is fragmented and domination of MGAT5-mediated glycosylation promotes plasma membrane expression of proteins via their strong interaction with Gal-3. This, in turn, facilitates PCa progression.

    Journal: Krimskii zhurnal eksperimental'noi i klinicheskoi meditsiny = Kryms'kyi zhurnal eksperymental'noi ta klinichnoi medytsyny = Crimean journal of experimental and clinical medicine

    Article Title: THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER

    doi:

    Figure Lengend Snippet: The working model of alcohol effect on N-glycosylation in PCa. In low aggressive PCa cells, pro-metastatic proteins are modified by MGAT3, which reduces their binding to the Gal-3, thus blocking their stabilization and retention at the cell surface. In alcohol-treated PCa cells, Golgi is fragmented and domination of MGAT5-mediated glycosylation promotes plasma membrane expression of proteins via their strong interaction with Gal-3. This, in turn, facilitates PCa progression.

    Article Snippet: The primary antibodies used were: a) rabbit polyclonal – MGAT3 (Abcam, ab103427), MGAT5 (Abcam, ab68595), and ST14 (Matriptase) (Abcam, ab28266); b) mouse monoclonal – E-cadherin (Abcam, ab1416), β-actin (Sigma, A2228), integrin alpha V + beta 6 (Abcam, ab77906), and giantin (Abcam, ab37266); c) mouse polyclonal – GM130 (Abcam, ab169276).

    Techniques: Modification, Binding Assay, Blocking Assay, Expressing